Composite

Part:BBa_K2372009:Design

Designed by: Marcel Chris Conrady   Group: iGEM17_Tuebingen   (2017-10-26)


pRha-RBS-BLS for controlled expression of beta-lactam synthetase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 157
    Illegal AgeI site found at 347
    Illegal AgeI site found at 527
    Illegal AgeI site found at 692
    Illegal AgeI site found at 1184
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 779
    Illegal BsaI site found at 1372


Design Notes

Like all Streptomyces genes BLS is GC-rich. IDT had problems synthesizing it and after cutting in half the synthesis worked but the Gibson Assembly was a pain.


Source

pRha and RBS are from the distribution kit, the BLS sequence is from Streptomyces clavuligerus.

References